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KMID : 0352519840210030169
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Korea Univercity Medical Journal
1984 Volume.21 No. 3 p.169 ~ p.177
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A Study on Lectin Histochemistry in Female Genital Organs
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尹汝霖/Youn, Yea-Lim
李大一/白承龍/Lee, Dale/Paik, Seung-Yong
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Abstract
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Recently, receptor specific lectins are being widely and usefully applied in the study of cell surface topography.
And binding of lectin by cell surface receptors can be demonstrated at three levels of sensitivity: by agglutination, immunohistology or electron microscopy and radioactive markers.
Especially, immunoenzyme histology using horseradish peroxidase as a conjugate has emerged as a powerful diagnostic tool with advantage of applicability to fixed tissue sections.
With the material obtained under controlled conditions, the specificity and sensitivity of immunoperoxidase staining have been reported to be comparable or superior to those of immunofluorescence.
However, reports on lectin immunoenzyme histology are fragmentary applying only to a single organ or tissue in a certain species.
Therefore, the author collected formalin fixed, paraffin embedded female genital organs of human, rabbit and mouse and applied 8 kinds of lectins showing different carbohydrate specificity to compare differences in lectin reactivity between interspecies or interorgan.
To demonstrate lectin reactivity, samples were stained by Avidin-Biotin-Complex method using biotinylated lectins obtained from Vector stain Lab. Inc.
The results obtained are as following:
1. Endometrial surface epithelium revealed specific binding sites for RCA I & PTA in mouse, UEA I in rabbit and Con -A in human and mouse.
2. Endometrial glandular epithelium showed specific binding sites for UEA I in rabbit, RCA I in mouse, and Con-A and PTA both in human and mouse.
3. Epithelium of the oviduct revealed specific binding sites for UEA I in rabbit, SBA in
rrouse, and Con-A both in rabbit and mouse.
4. Luteal cells of the ovary exhibited specific binding sites for DBA in rabbit, WGA in human and mouse, and RCA I in human and rabbit.
5. Granulosa cell showed specific binding sites only for Con-A in mouse.
6. Theca cell revealed specific binding sites for WGA and PNA in human, PTA in human and rabbit.
7. Zona pellucida disclosed specific binding sites for RCA I in rabbit, WGA and DBA in mouse.
As far as normal cells of female reproductive organs of human, rabbit and mouse are concerned, above findings suggest that the pattern of carbohydrate consitituent of the cell membrane are substantially different between interspecies and interorgan.
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KEYWORD
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